Crafting Skilled Movement: Early Molecular Specification in Subcerebral Projection Neurons

Skilled motor function is controlled by subcerebral projection neurons (SCPN), which extend axons from the cerebral cortex to appropriate subcerebral targets, innervating either the brainstem (cortico-brainstem projection neurons, CBN) or spinal cord (corticospinal projection neurons, CSN). 

Special guest speakers, Payal Patel and Julia Kaiser, are joining NanoCellect to present their recent work using the WOLF Cell Sorter to purify before gene expression analysis for molecular specification of cortico-brainstem vs. corticospinal projection neurons, and what it suggests for subcerebral projection neuron axon targeting early in development.

This webinar highlights:

• The brainstem-spinal transition zone, which is molecularly established early in development
• The experimental scheme of purifying whole cell SCPN and CSN subpopulations using the WOLF Cell Sorter for single cell RNA-sequencing