Techniques for single-cell iPSC cloning in CRISPR/Cas9 workflows.

Still hand-picking iPSC colonies? Consider gentle single-cell sorting. Join us for our mini webinar highlighting tips and techniques for sorting iPSC. We review:

    • Example workflows for fluorescence targeted CRISPR/Cas9 screening
    • Techniques and challenges when preparing iPSC for sorting
    • How gentle single-cell sorting will reduce your iPSC workload & simplify clonal selection

We discuss methods for sorting CRISPR/Cas9-edited iPSC. This discussion showcases work from our collaborators at the Human Stem Cell Core at Baylor College of Medicine, among others, to convey how using the WOLF® cell sorter and N1 single-cell dispenser has enabled recent advancements.