Generate High-Quality Monoclonal Cell Lines—Faster and Gentler

Creating stable, high-producing monoclonal cell lines shouldn’t take weeks of trial and error. The NanoCellect WOLF G2^® Cell Sorter streamlines single-cell cloning with a gentle, sterile microfluidic system that maximizes outgrowth, viability, and productivity.

Traditional methods like limiting dilution or droplet-based FACS expose cells to high pressure and shear stress—often compromising viability. In contrast, the WOLF’s low-pressure (<2 PSI) microfluidics and disposable sterile cartridges deliver single-cell sorting and dispensing with high clonal outgrowth and cell health.

Proven performance across multiple studies:

• Up to 8× more monoclonal colonies than limiting dilution, with nearly 2× higher expression levels — WOLF Exceeds Limiting Dilution Cell Cloning Efficiency

• >90% single-cell plating efficiency and >87% monoclonal outgrowth in CHO cells — Single-Cell Cloning for Cell Line Development

• Improvement in outgrowth of sensitive engineered cells vs. traditional FACS — Isolate Antibody-Secreting CHO Cells for Single-Cell Cloning

Whether you’re developing therapeutic antibodies, CRISPR-engineered models, or recombinant proteins, the WOLF G2 helps your team accelerate cell line development while preserving sterility, viability, and reproducibility.

Let's discuss how gentle cell sorting can empower
your research!