Hi there,
I'm following up on my email about the WOLF G2 Cell Sorter.
You might be a bit skeptical (especially if you've never heard of us before!) so I want to share an application note on how the WOLF has been used in enrichment of memory B Cells for antibody discovery by successfully enriching live CD3- CD8-CD14-CD27+ cells from an average pre-sort target population of 2.4 ± 1.2% to a post-sort target of 81.5 ± 0.8% when using a starting sorting concentration of 1.0 x 106 cells/ mL.
The WOLF system gently sorts cells at <2 PSI, removing dead cells and debris for an enriched cell population that is healthy and viable with intact cell membranes, ready to be used in downstream workflows.